Stem Cells http://www.stemcellsportal.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

First published online June 28, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
2007-0019v1
25/10/2448    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fukada, S. i.
Right arrow Articles by Takeda, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fukada, S. i.
Right arrow Articles by Takeda, S.
Submitted on January 8, 2007
Accepted on June 19, 2007

TISSUE-SPECIFIC STEM CELLS

Molecular Signature of Quiescent Satellite Cells In Adult Skeletal Muscle

So ichiro Fukada 1, Akiyoshi Uezumi 1, Madoka Ikemoto 1, Satoru Masuda 1, Masashi Segawa 2, Naoki Tanimura 3, Hiroshi Yamamoto 2, Yuko Miyagoe-Suzuki 1*, Shin'ichi Takeda 1

1 Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan
2 Department of Immunology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan
3 Bio and Nano Technologies, Science and Technology Division, Mizuho Information & Research Institute, Inc., 2-3 Kanda-Nishikicho, Chiyoda-ku, Tokyo 101-8443, Japan

* To whom correspondence should be addressed. E-mail: miyagoe{at}ncnp.go.jp.


  Abstract

Skeletal muscle satellite cells play key roles in postnatal muscle growth and regeneration. To study molecular regulation of satellite cells, we directly prepared satellite cells from 8- to 12-week-old C57BL/6 mice and performed genome-wide gene expression analysis. Compared with activated/cycling satellite cells, 507 genes were highly up-regulated in quiescent satellite cells. These include negative regulators of cell cycle and myogenic inhibitors. Gene set enrichment analysis revealed that quiescent satellite cells preferentially express the genes involved in cell-cell adhesion, regulation of cell growth, formation of extracellular matrix, copper and iron homeostasis, and lipid transportation. Furthermore, RT-PCR on differentially expressed genes confirmed that calcitonin receptor (CTR) was exclusively expressed in dormant satellite cells, but not in activated satellite cells. In addition, CTR mRNA is hardly detected in non-myogenic cells. Therefore, we next examined the expression of CTR in vivo. CTR was specifically expressed on quiescent satellite cells, but the expression was not found on activated/proliferating satellite cells during muscle regeneration. CTR-positive cells reappeared at the rim of regenerating myofibers in later stages of muscle regeneration. Calcitonin stimulation delayed the activation of quiescent satellite cells. Our data provide roles of CTR in quiescent satellite cells and a solid scaffold to further dissect molecular regulation of satellite cells.

Key Words. FACS, Microarray, Quiescence, Muscle satellite cells, Calcitonin receptor




This article has been cited by other articles:


Home page
 Genes Dev.Home page
M. Lagha, J. D. Kormish, D. Rocancourt, M. Manceau, J. A. Epstein, K. S. Zaret, F. Relaix, and M. E. Buckingham
Pax3 regulation of FGF signaling affects the progression of embryonic progenitor cells into the myogenic program
Genes & Dev., July 1, 2008; 22(13): 1828 - 1837.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.stemcellsportal.com/
Copyright © 2007 by AlphaMed Press.