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First published online May 24, 2007
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2007-0080v1
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Submitted on February 9, 2007
Accepted on May 11, 2007

MEETING REPORT

Isolation and Characterization of Neural Crest Progenitors from Adult Dorsal Root Ganglia

Hong Yun Li 1, Evonne Hwee Min Say 2, Xin Fu Zhou 2*

1 Department of Human Physiology, Flinders University, GPO Box 2100, Adelaide 5001, Australia. Department of CNS Trauma Rehabilitation, Research Institute of Surgery, Daping Hospital, Chongqing, 400042, P.R. China.
2 Department of Human Physiology, Flinders University, GPO Box 2100, Adelaide 5001, Australia.

* To whom correspondence should be addressed. E-mail: xin-fu.zhou{at}flinders.edu.au.


  Abstract

After peripheral nerve injury, the number of sensory neurons in the adult dorsal root ganglia (DRG) is initially reduced but recovers to a normal level several months later. The mechanisms underlying the neuronal recovery after injury are not clear. Here we showed that in the DRG explants culture, a subpopulation of cells emigrated out from adult rat DRG expressed nestin and p75NTR, and formed clusters and spheres. They differentiated into neurons, glia and smooth muscle cells in the presence or absence of serum and formed secondary and tertiary neurospheres in cloning assays. Molecular expression analysis demonstrated the characteristics of neural crest progenitors and their potentials of neuronal differentiation by expressing a set of well-defined genes related to adult stem cells niches and neuronal fate-decision. Under the influence of neurotrophic factors, some of these progenitors gave rise to neuropeptide-expressing cells and P0 expressing Schwann cells. By a BrdU chasing study, we showed that these progenitors likely originate from satellite glial cells. Our study suggests that a subpopulation of glia in adult DRG are likely progenitors for neurons and glia and may play a role in neurogenesis after nerve injury.

Key Words. neural crest progenitors, dorsal root ganglia, neurogenesis, explants culture, satellite glial cells, immunocytochemistry




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S. Thomas, M. Thomas, P. Wincker, C. Babarit, P. Xu, M. C. Speer, A. Munnich, S. Lyonnet, M. Vekemans, and H. C. Etchevers
Human neural crest cells display molecular and phenotypic hallmarks of stem cells
Hum. Mol. Genet., November 1, 2008; 17(21): 3411 - 3425.
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