Submitted on February 8, 2007
Accepted on May 4, 2007
STEM CELL GENETICS AND GENOMICS: CONCISE REVIEW
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Trends in Stem Cell Proteomics
Hossein Baharvand 1*,
Ali Fathi 1,
Dennis van Hoof 2,
Ghasem Hosseini Salekdeh 3*
1 Department of Stem Cells, Royan Institute, Tehran, Iran
2 Hubrecht Laboratory, Netherlands Institute of Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; Department of Biomolecular Mass Spectrometry, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
3 Department of Physiology and Proteomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran.
* To whom correspondence should be addressed. E-mail: Baharvand50{at}yahoo.com.
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Abstract |
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Gene expression analyses of stem cells (SCs) will help to uncover or further define signaling pathways and molecular mechanisms involved in the maintenance of self-renewal, pluripotency, and/or multipotency. In recent years, proteomic approaches have produced a wealth of data identifying proteins and mechanisms involved in SC proliferation and differentiation. Although many proteomics techniques have been developed and improved in peptide and protein separation as well as mass spectrometry, several important issues including sample heterogeneity, post-translational modifications, protein-protein interaction, and high throughput quantification of hydrophobic and low abundant proteins still remain to be addressed and require further technical optimization. The following review summarizes the methodologies used and the information gathered with proteome analyses of SCs, and discusses biological and technical challenges for proteomic study of SCs.
Key Words.
Stem cells, Heterogeneity, Proteomics, Mass spectrometry, Post-translational modification, electrophoresis, Quantitative proteomics, Secretomics, Two-dimensional electrophoresis.
