Submitted on March 13, 2007
Accepted on April 27, 2007
TRANSLATIONAL AND CLINICAL RESEARCH
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Extensive Studies on Perfusion Method Plus Intra-Bone Marrow-Bone Marrow Transplantation Using Cynomolgus Monkeys
MUNEO INABA 1,
YASUSHI ADACHI 2,
HIROKO HISHA 2,
NAOKI HOSAKA 1,
MASAHIKO MAKI 3,
YUSUKE UEDA 4,
YASUSHI KOIKE 3,
TAKESHI MIYAKE 3,
JUNICHI FUKUI 3,
YUNZE CUI 5,
HIROMI MUKAIDE 3,
NAOKO KOIKE 3,
MARIKO OMAE 3,
TOMOMI MIZOKAMI 3,
AKIO SHIGEMATSU 3,
YUTAKU SAKAGUCHI 3,
MASANOBU TSUDA 3,
SATOSHI OKAZAKI 3,
XIAOLI WANG 3,
QING LI 3,
AKIHISA NISHIDA 3,
YUGO ANDO 3,
KEQUAN GUO 3,
CHANGYE SONG 3,
WENHAO CUI 3,
WEI FENG 3,
JUNKO KATOU 3,
KATUYUKI SADO 6,
SHUJI NAKAMURA 6,
SUSUMU IKEHARA 1*
1 From the First Department of Pathology, Transplantation Center, Regeneration Research Center for Intractable Diseases, Center for Cancer Therapy, Kansai Medical University, Osaka, Japan
2 Transplantation Center, Regeneration Research Center for Intractable Diseases, Center for Cancer Therapy, Kansai Medical University, Osaka, Japan
3 From the First Department of Pathology, Kansai Medical University, Osaka, Japan
4 From the First Department of Pathology, Department of Orthopedic Surgery, Kansai Medical University, Osaka, Japan
5 From the First Department of Pathology, Kansai Medical University, Osaka, Japan, JIMRO Co., Ltd. (JIMRO), Takasaki, Gunma, Japan
6 JIMRO Co., Ltd. (JIMRO), Takasaki, Gunma, Japan
* To whom correspondence should be addressed. E-mail: ikehara{at}takii.kmu.ac.jp.
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Abstract |
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The collection of bone marrow cells (BMCs) using a perfusion method (PM) has been advantageous not only because of the low contamination of BMCs with T cells from the peripheral blood but also the enrichment of stromal cells, which support hemopoiesis. Before the application of this new method to humans, its safety needed to be confirmed using cynomolgus monkeys. We therefore performed the perfusion method on more than 100 cynomolgus monkeys, using the long bones (such as the humerus and femur) and also the iliac bones (for human application); in the more than 150 trials to date, there have been no accidental deaths. Furthermore, the technical safety of a new method for the intra-bone marrow (IBM) injection of BMCs (termed IBM-BMT) has also been confirmed using 30 monkeys.
Key Words.
Perfusion method, Intra-bone marrow-bone marrow transplantation, Cynomolgus monkey, Colony-forming unit in culture, Stromal cell
