Stem Cells http://www.stemcellsportal.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

First published online October 18, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
2007-0494v1
26/1/55    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Author home page(s):
Su Chun Zhang
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, D.
Right arrow Articles by Zhang, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, D.
Right arrow Articles by Zhang, S. C.
Submitted on June 21, 2007
Accepted on October 9, 2007

EMBRYONIC STEM CELLS

Human Embryonic Stem Cell-derived Dopaminergic Neurons Reverse Functional Deficit in Parkinsonian Rats

Dali Yang 1, Zhi Jian Zhang 1, Michael Oldenburg 1, Melvin Ayala 1, Su Chun Zhang 1*

1 Departments of Anatomy and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin-Madison, Madison, Wisconsin, WI 53705, USA.

* To whom correspondence should be addressed. E-mail: zhang{at}waisman.wisc.edu.


  Abstract

We show that human embryonic stem cell (hESC)-derived dopaminergic neurons survived transplantation to the neurotoxin 6-hydroxydopamine (6-OHDA) lesioned rat striatum, and in combination with the cells newly differentiated from their progenitors, contributed to locomotive function recovery at 5 months. The animal behavioral improvement was correlated to the dopamine neurons present in the graft. Although the donor cells contained forebrain and midbrain dopamine neurons, the dopamine neurons present in the graft mainly exhibited a midbrain, or nigra phenotype, suggesting the importance of midbrain dopamine neurons in functional repair. Furthermore, progenies of grafted cells were neurons and glia with greatly diminished mitotic activity by 5 months. Thus, the in vitro produced human dopamine neurons can functionally engraft in the brain.

______________________________________________________________

D. Yang and Z-J Zhang contributed equally to this article.

 Key Words. Dopaminergic neurons, Cell therapy, Transplantation, Parkinson's disease, Rats




This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
G. W. Yeo, N. Coufal, S. Aigner, B. Winner, J. A. Scolnick, M. C.N. Marchetto, A. R. Muotri, C. Carson, and F. H. Gage
Multiple layers of molecular controls modulate self-renewal and neuronal lineage specification of embryonic stem cells
Hum. Mol. Genet., April 15, 2008; 17(R1): R67 - R75.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Wernig, J.-P. Zhao, J. Pruszak, E. Hedlund, D. Fu, F. Soldner, V. Broccoli, M. Constantine-Paton, O. Isacson, and R. Jaenisch
Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease
PNAS, April 15, 2008; 105(15): 5856 - 5861.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.peprotech.com/
Copyright © 2007 by AlphaMed Press.