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EMBRYONIC STEM CELLS: CHARACTERIZATION SERIES

Derivation of a Xeno-Free Human Embryonic Stem Cell Line

^aCellartis AB, Göteborg, Sweden;
^bStem Cell Center, Lund University, Lund, Sweden;
^cReproductive Medicine, Department of Obstetrics and Gynaecology, Institute for Health of Women and Children, Sahlgrenska Academy, Göteborg, Sweden

Key Words. Human embryonic stem cell • Human serum • Human feeders • Clinical therapies

Correspondence: Henrik Semb, Ph.D., Stem Cell Center, Lund University, BMC, B10, SE-221-84 Lund, Sweden. Telephone: 46-46-222-31-59; Fax: 46-46-222-36-00; e-mail: henrik.semb{at}med.lu.se

Received March 6, 2006; accepted for publication May 23, 2006.
First published online in STEM CELLS EXPRESS   June 1, 2006.



Elimination of all animal material during both the derivation and long-term culture of human embryonic stem cells (hESCs) is necessary prior to future application of hESCs in clinical cell therapy. The potential consequences of transplanting xeno-contaminated hESCs into patients, such as an increased risk of graft rejection [STEM CELLS 2006;24:221–229] and the potential transfer of nonhuman pathogens, make existing hESC lines unsuitable for clinical applications. To avoid xeno-contamination during derivation and culture of hESCs, we first developed a xeno-free medium supplemented with human serum, which supports long-term (>50 passages) culture of hESCs in an undifferentiated state. To enable derivation of new xeno-free hESCs, we also established xeno-free human foreskin fibroblast feeders and replaced immunosurgery, which involves the use of guinea pig complement, with a modified animal-product-free derivation procedure. Here, we report the establishment and characterization (>20 passages) of a xeno-free pluripotent diploid normal hESC line, SA611.

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