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First published online August 31, 2006
Stem Cells Vol. 24 No. 12 December 2006, pp. 2851 -2857
doi:10.1634/stemcells.2006-0399; www.StemCells.com
© 2006 AlphaMed Press

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TISSUE-SPECIFIC STEM CELLS

Neurosphere Assays: Growth Factors and Hormone Differences in Tumor and Nontumor Studies

Kaisorn Chaichana, Grettel Zamora-Berridi, Joaquin Camara-Quintana, Alfredo Quiñones-Hinojosa

Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Key Words. Neurosphere assay • Neural stem cells • Brain tumor stem cells

Correspondence: Alfredo Quiñones-Hinojosa, M.D., The Johns Hopkins Hospital, Department of Neurosurgery, Johns Hopkins University, CRB II, 1550 Orleans Street, Room 253, Baltimore, Maryland 21231, USA. Telephone: 410-502-3204; Fax: 410-502-5559; e-mail: aquinon2{at}jhmi.edu

Received June 29, 2006; accepted for publication August 22, 2006.
First published online in STEM CELLS EXPRESS   August 31, 2006.



The "no new neuron" dogma that the brain is quiescent throughout adult life has been challenged by the discovery of cells with stem cell-like qualities of self-renewal and multipotency in the subventricular zone of the lateral ventricles and the dentate gyrus of the hippocampus in adults. This self-renewing capacity also makes these neural stem cells a possible source of brain tumors, which was supported by the discovery of self-sustaining brain tumor stem-like cells in cancers such as glioblastoma multiforme. Neurosphere assays are the standard for studying these stem-like cells in both normal and cancer tissues. Despite the importance of these assays, there is no standardized protocol to allow for a comparison of results because several studies use different growth factors and hormones at different concentrations. The primary objective of this study is to review the literature for both nontumor and tumor studies to assess their respective neurosphere assay components. We found significant variation in assay components, namely hormones and growth factors, as well as their respective concentrations. This illustrates the need for a standardized protocol to allow proper comparison among studies and a better assessment of the effects of different factors.




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[Abstract] [Full Text] [PDF]


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