HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 2005-0204v1 24/2/266    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lacham-Kaplan, O. Articles by Trounson, A. Search for Related Content PubMed PubMed Citation Articles by Lacham-Kaplan, O. Articles by Trounson, A.

EMBRYONIC STEM CELLS

Testicular Cell Conditioned Medium Supports Differentiation of Embryonic Stem Cells into Ovarian Structures Containing Oocytes

Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Australia

Key Words. Embryonic stem cells • Germ stem cells • Testicular cell cultures • Conditioned medium • Oocytes

Correspondence: Orly Lacham-Kaplan, Ph.D., Monash Immunology and Stem Cell Laboratories, Monash University, Wellington Rd., Clayton, Australia 3165. Telephone: 61-3-99050781; Fax: 61-3-99050680; e-mail: Orly.Lacham-kaplan{at}med.monash.edu.au

Previous reports and the current study have found that germ cell precursor cells appear in embryoid bodies (EBs) formed from mouse embryonic stem cells as identified by positive expression of specific germ cell markers such as Oct-3/4, Mvh, c-kit, Stella, and DAZL. We hypothesized that if exposed to appropriate growth factors, the germ cell precursor cells within the EBs would differentiate into gametes. The source for growth factors used in the present study is conditioned medium collected from testicular cell cultures prepared from the testes of newborn males. Testes at this stage of development contain most growth factors required for the transformation of germ stem cells into differentiated gametes. When EBs were cultured in the conditioned medium, they developed into ovarian structures, which contained putative oocytes. The oocytes were surrounded by one to two layers of flattened cells and did not have a visible zona pellucida. However, oocyte-specific markers such as Fig- and ZP3 were found expressed by the ovarian structures. The production of oocytes using this method is repeatable and reliable and may be applicable to other mammalian species, including the human.

This article has been cited by other articles:

				S. Ziebe, P. Devroey, and on behalf of the State of the ART 2007 Workshop Gr Assisted reproductive technologies are an integrated part of national strategies addressing demographic and reproductive challenges Hum. Reprod. Update, November 1, 2008; 14(6): 583 - 592. [Abstract] [Full Text] [PDF]

				F. D. West, D. W. Machacek, N. L. Boyd, K. Pandiyan, K. R. Robbins, and S. L. Stice Enrichment and Differentiation of Human Germ-Like Cells Mediated by Feeder Cells and Basic Fibroblast Growth Factor Signaling Stem Cells, November 1, 2008; 26(11): 2768 - 2776. [Abstract] [Full Text] [PDF]

				A. J. Childs, P. T.K. Saunders, and R. A. Anderson Modelling germ cell development in vitro Mol. Hum. Reprod., September 1, 2008; 14(9): 501 - 511. [Abstract] [Full Text] [PDF]

				H.-J. Lee, K. Selesniemi, Y. Niikura, T. Niikura, R. Klein, D. M. Dombkowski, and J. L. Tilly Bone Marrow Transplantation Generates Immature Oocytes and Rescues Long-Term Fertility in a Preclinical Mouse Model of Chemotherapy-Induced Premature Ovarian Failure J. Clin. Oncol., August 1, 2007; 25(22): 3198 - 3204. [Abstract] [Full Text] [PDF]

				C. E. Gargett Review Article: Stem Cells in Human Reproduction Reproductive Sciences, July 1, 2007; 14(5): 405 - 424. [Abstract] [PDF]

				G. Q. Daley Gametes from Embryonic Stem Cells: A Cup Half Empty or Half Full? Science, April 20, 2007; 316(5823): 409 - 410. [Abstract] [Full Text] [PDF]

				H.-F. Chen, H.-C. Kuo, C.-L. Chien, C.-T. Shun, Y.-L. Yao, P.-L. Ip, C.-Y. Chuang, C.-C. Wang, Y.-S. Yang, and H-N. Ho Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation Hum. Reprod., February 1, 2007; 22(2): 567 - 577. [Abstract] [Full Text] [PDF]

				B. Aflatoonian and H. Moore Germ cells from mouse and human embryonic stem cells. Reproduction, November 1, 2006; 132(5): 699 - 707. [Abstract] [Full Text] [PDF]

				I. Novak, D. A. Lightfoot, H. Wang, A. Eriksson, E. Mahdy, and C. Hoog Mouse Embryonic Stem Cells Form Follicle-Like Ovarian Structures but Do Not Progress Through Meiosis Stem Cells, August 1, 2006; 24(8): 1931 - 1936. [Abstract] [Full Text] [PDF]