| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
TISSUE-SPECIFIC STEM CELLS |
a Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden-Württemberg–Hessen, Mannheim, Germany;
b Department of Orthopaedic Surgery of the University Hospital Mannheim, University of Heidelberg, Faculty of Clinical Medicine, Mannheim, Germany
Key Words. Mesenchymal stem cells • Bone marrow • Umbilical cord blood • Adipose tissue • Comparative analysis • Multilineage differentiation
Correspondence: Karen Bieback, Ph.D., Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden-Württemberg – Hessen, University of Heidelberg, Faculty of Clinical Medicine Mannheim, Friedrich-Ebert-Straße 107, D-68167 Mannheim, Germany. Telephone: +49-621-3706-8216; Fax: +49-621-3706-851; e-mail: k.bieback{at}itima.blutspende.de
Received July 28, 2005;
accepted for publication December 21, 2005.
Mesenchymal stem cells (MSCs) represent a promising tool for new clinical concepts in supporting cellular therapy. Bone marrow (BM) was the first source reported to contain MSCs. However, for clinical use, BM may be detrimental due to the highly invasive donation procedure and the decline in MSC number and differentiation potential with increasing age. More recently, umbilical cord blood (UCB), attainable by a less invasive method, was introduced as an alternative source for MSCs. Another promising source is adipose tissue (AT). We compared MSCs derived from these sources regarding morphology, the success rate of isolating MSCs, colony frequency, expansion potential, multiple differentiation capacity, and immune phenotype. No significant differences concerning the morphology and immune phenotype of the MSCs derived from these sources were obvious. Differences could be observed concerning the success rate of isolating MSCs, which was 100% for BM and AT, but only 63% for UCB. The colony frequency was lowest in UCB, whereas it was highest in AT. However, UCB-MSCs could be cultured longest and showed the highest proliferation capacity, whereas BM-MSCs possessed the shortest culture period and the lowest proliferation capacity. Most strikingly, UCB-MSCs showed no adipogenic differentiation capacity, in contrast to BM- and AT-MSCs. Both UCB and AT are attractive alternatives to BM in isolating MSC: AT as it contains MSCs at the highest frequency and UCB as it seems to be expandable to higher numbers.
This article has been cited by other articles:
|
|
 |
|
  D. J. Weiss, J. K. Kolls, L. A. Ortiz, A. Panoskaltsis-Mortari, and D. J. Prockop Stem Cells and Cell Therapies in Lung Biology and Lung Diseases Proceedings of the ATS, July 15, 2008; 5(5): 637 - 667. [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Rebelatto, A. M. Aguiar, M. P. Moretao, A. C. Senegaglia, P. Hansen, F. Barchiki, J. Oliveira, J. Martins, C. Kuligovski, F. Mansur, et al. Dissimilar Differentiation of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, and Adipose Tissue Experimental Biology and Medicine, July 1, 2008; 233(7): 901 - 913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Izadpanah, D. Kaushal, C. Kriedt, F. Tsien, B. Patel, J. Dufour, and B. A. Bunnell Long-term In vitro Expansion Alters the Biology of Adult Mesenchymal Stem Cells Cancer Res., June 1, 2008; 68(11): 4229 - 4238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Rider, C. Dombrowski, A. A. Sawyer, G. H. B. Ng, D. Leong, D. W. Hutmacher, V. Nurcombe, and S. M. Cool Autocrine Fibroblast Growth Factor 2 Increases the Multipotentiality of Human Adipose-Derived Mesenchymal Stem Cells Stem Cells, June 1, 2008; 26(6): 1598 - 1608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Dimitrov, T Timeva, D Kyurkchiev, M Stamenova, A Shterev, P Kostova, V Zlatkov, I Kehayov, and S Kyurkchiev Characterization of clonogenic stromal cells isolated from human endometrium Reproduction, April 1, 2008; 135(4): 551 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Troyer and M. L. Weiss Concise Review: Wharton's Jelly-Derived Cells Are a Primitive Stromal Cell Population Stem Cells, March 1, 2008; 26(3): 591 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gandia, A. Arminan, J. M. Garcia-Verdugo, E. Lledo, A. Ruiz, M D. Minana, J. Sanchez-Torrijos, R. Paya, V. Mirabet, F. Carbonell-Uberos, et al. Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction Stem Cells, March 1, 2008; 26(3): 638 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Magatti, S. De Munari, E. Vertua, L. Gibelli, G. S. Wengler, and O. Parolini Human Amnion Mesenchyme Harbors Cells with Allogeneic T-Cell Suppression and Stimulation Capabilities Stem Cells, January 1, 2008; 26(1): 182 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Secco, E. Zucconi, N. M. Vieira, L. L.Q. Fogaca, A. Cerqueira, M. D. F. Carvalho, T. Jazedje, O. K. Okamoto, A. R. Muotri, and M. Zatz Multipotent Stem Cells from Umbilical Cord: Cord Is Richer than Blood! Stem Cells, January 1, 2008; 26(1): 146 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Bai, J. Ma, Z. Pan, Y.-H. Song, S. Freyberg, Y. Yan, D. Vykoukal, and E. Alt Electrophysiological properties of human adipose tissue-derived stem cells Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1539 - C1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Trzaska, E. V. Kuzhikandathil, and P. Rameshwar Specification of a Dopaminergic Phenotype from Adult Human Mesenchymal Stem Cells Stem Cells, November 1, 2007; 25(11): 2797 - 2808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wagner, C. Roderburg, F. Wein, A. Diehlmann, M. Frankhauser, R. Schubert, V. Eckstein, and A. D. Ho Molecular and Secretory Profiles of Human Mesenchymal Stromal Cells and Their Abilities to Maintain Primitive Hematopoietic Progenitors Stem Cells, October 1, 2007; 25(10): 2638 - 2647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Park, K. H. Jung, S. H. Kim, K. S. Kim, M. R. Choi, Y. Kim, and Y. G. Chai Functional Expression of Ion Channels in Mesenchymal Stem Cells Derived from Umbilical Cord Vein Stem Cells, August 1, 2007; 25(8): 2044 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Schafer, R. Kehlbach, J. Wiskirchen, R. Bantleon, J. Pintaske, B. R. Brehm, A. Gerber, H. Wolburg, C. D. Claussen, and H. Northoff Transferrin Receptor Upregulation: In Vitro Labeling of Rat Mesenchymal Stem Cells with Superparamagnetic Iron Oxide Radiology, August 1, 2007; 244(2): 514 - 523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kucerova, V. Altanerova, M. Matuskova, S. Tyciakova, and C. Altaner Adipose Tissue-Derived Human Mesenchymal Stem Cells Mediated Prodrug Cancer Gene Therapy Cancer Res., July 1, 2007; 67(13): 6304 - 6313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Kunter, S. Rong, P. Boor, F. Eitner, G. Muller-Newen, Z. Djuric, C. R. van Roeyen, A. Konieczny, T. Ostendorf, L. Villa, et al. Mesenchymal Stem Cells Prevent Progressive Experimental Renal Failure but Maldifferentiate into Glomerular Adipocytes J. Am. Soc. Nephrol., June 1, 2007; 18(6): 1754 - 1764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kocaoemer, S. Kern, H. Kluter, and K. Bieback Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue Stem Cells, May 1, 2007; 25(5): 1270 - 1278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schaffler and C. Buchler Concise Review: Adipose Tissue-Derived Stromal Cells--Basic and Clinical Implications for Novel Cell-Based Therapies Stem Cells, April 1, 2007; 25(4): 818 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Jeong, K.-M. Ko, S. Bae, C.-J. Jeon, G. Young Koh, and H. Kim Genome-Wide Differential Gene Expression Profiling of Human Bone Marrow Stromal Cells Stem Cells, April 1, 2007; 25(4): 994 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Boquest, A. Noer, A. L. Sorensen, K. Vekterud, and P. Collas CpG Methylation Profiles of Endothelial Cell-Specific Gene Promoter Regions in Adipose Tissue Stem Cells Suggest Limited Differentiation Potential Toward the Endothelial Cell Lineage Stem Cells, April 1, 2007; 25(4): 852 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. Guillot, C. Gotherstrom, J. Chan, H. Kurata, and N. M. Fisk Human First-Trimester Fetal MSC Express Pluripotency Markers and Grow Faster and Have Longer Telomeres Than Adult MSC Stem Cells, March 1, 2007; 25(3): 646 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Noer, A. L. Sorensen, A. C. Boquest, and P. Collas Stable CpG Hypomethylation of Adipogenic Promoters in Freshly Isolated, Cultured, and Differentiated Mesenchymal Stem Cells from Adipose Tissue Mol. Biol. Cell, August 1, 2006; 17(8): 3543 - 3556. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
