| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
EMBRYONIC STEM CELLS |
a Department of Pediatrics, Graduate School of Medicine,
b Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences,
f Department of Development and Differentiation, Institute for Frontier Medical Science, Kyoto University, Kyoto, Japan;
c Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA;
d Research Center for Animal Life Science, Shiga University of Medical Science, Ohtsu, Japan;
e Division of Genetics, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Key Words. Embryonic stem cell • Primate • Hemangioblast • Vascular endothelial growth factor
Correspondence: Tatsutoshi Nakahata, M.D., Ph.D., Department of Pediatrics, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. Telephone: 81-75-751-3290; Fax: 81-75-752-2361; e-mail: tnakaha{at}kuhp.kyoto-u.ac.jp
Received April 11, 2005;
accepted for publication December 15, 2005.
We identified intermediate-stage progenitor cells that have the potential to differentiate into hematopoietic and endothelial lineages from nonhuman primate embryonic stem (ES) cells. Sequential fluorescence-activated cell sorting and immunostaining analyses showed that when ES cells were cultured in an OP9 coculture system, both lineages developed after the emergence of two hemoangiogenic progenitor-bearing cell fractions, namely, vascular endothelial growth factor receptor (VEGFR)-2high CD34– and VEGFR-2high CD34+ cells. Exogenous vascular endothelial growth factor increased the proportion of VEGFR-2high cells, particularly that of VEGFR-2high CD34+ cells, in a dose-dependent manner. Although either population of VEGFR-2high cells could differentiate into primitive and definitive hematopoietic cells (HCs), as well as endothelial cells (ECs), the VEGFR-2high CD34+ cells had greater hemoangiogenic potential. Both lineages developed from VEGFR-2high CD34–or VEGFR-2high CD34+ precursor at the single-cell level, which strongly supports the existence of hemangioblasts in these cell fractions. Thus, this culture system allows differentiation into the HC and EC lineages to be defined by surface markers. These observations should facilitate further studies both on early developmental processes and on regeneration therapies in human.
This article has been cited by other articles:
|
|
 |
|
  G. Shinoda, K. Umeda, T. Heike, M. Arai, A. Niwa, F. Ma, H. Suemori, H. Y. Luo, D. H. K. Chui, R. Torii, et al. {alpha}4-Integrin+ endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells Blood, March 15, 2007; 109(6): 2406 - 2415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Rajesh, N. Chinnasamy, S. M. Mitalipov, D. P. Wolf, I. Slukvin, J. A. Thomson, and A. F. Shaaban Differential Requirements for Hematopoietic Commitment Between Human and Rhesus Embryonic Stem Cells Stem Cells, February 1, 2007; 25(2): 490 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Umeda, T. Heike, M. Nakata-Hizume, A. Niwa, M. Arai, G. Shinoda, F. Ma, H. Suemori, H. Y. Luo, D. H. K. Chui, et al. Sequential Analysis of {alpha}- and {beta}-Globin Gene Expression During Erythropoietic Differentiation from Primate Embryonic Stem Cells Stem Cells, December 1, 2006; 24(12): 2627 - 2636. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
