HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 2007-0054v1 25/7/1737    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ponte, A. L. Articles by Domenech, J. Search for Related Content PubMed PubMed Citation Articles by Ponte, A. L. Articles by Domenech, J.

TISSUE-SPECIFIC STEM CELLS

The In Vitro Migration Capacity of Human Bone Marrow Mesenchymal Stem Cells: Comparison of Chemokine and Growth Factor Chemotactic Activities

^aLaboratoire d'Hématopoïèse, Institut National de la Santé et de la Recherche Médicale ERI5, IFR135 Université François Rabelais, Tours, France;
^bEFS Centre-Atlantique, Tours, France;
^cCHRU de Tours, Tours, France

Key Words. Bone marrow • Mesenchymal stem cell • In vitro migration • Growth factors • Chemokines • Tumor necrosis factor

Correspondence: Jorge Domenech M.D., Ph.D., Laboratoire d'Hématopoïèse, Inserm-ESPRI-EA3855, Faculté de Médecine, 10 boulevard Tonnellé, 37 032 Tours Cedex, France. Telephone: 33-247-47-47-21; Fax: 33-247-47-69-34; e-mail: domenech{at}med.univ-tours.fr

Received January 24, 2007; accepted for publication March 20, 2007.
First published online in STEM CELLS EXPRESS   March 29, 2007.



Adult bone marrow (BM)-derived stem cells, including hematopoietic stem cells (HSCs) and MSCs, represent an important source of cells for the repair of a number of damaged tissues. In contrast to HSCs, the soluble factors able to induce MSC migration have not been extensively studied. In the present work, we compared the in vitro migration capacity of human BM-derived MSCs, preincubated or not with the inflammatory cytokines interleukin 1ß (IL1ß) and tumor necrosis factor (TNF), in response to 16 growth factors (GFs) and chemokines. We show that BM MSCs migrate in response to many chemotactic factors. The GFs platelet-derived growth factor-AB (PDGF-AB) and insulin-like growth factor 1 (IGF-1) are the most potent, whereas the chemokines RANTES, macrophage-derived chemokine (MDC), and stromal-derived factor-1 (SDF-1) have limited effect. Remarkably, preincubation with TNF leads to increased MSC migration toward chemokines, whereas migration toward most GFs is unchanged. Consistent with these results, BM MSCs express the tyrosine kinase receptors PDGF-receptor (R) , PDGF-Rß, and IGF-R, as well as the RANTES and MDC receptors CCR2, CCR3, and CCR4 and the SDF-1 receptor CXCR4. TNF increases CCR2, CCR3, and CCR4 expression (as opposed to that of CXCR4), together with RANTES membrane binding. These data indicate that the migration capacity of BM MSCs is under the control of a large range of receptor tyrosine kinase GFs and CC and CXC chemokines. Most chemokines are more effective on TNF-primed cells. Our results suggest that the mobilization of MSCs and their subsequent homing to injured tissues may depend on the systemic and local inflammatory state.

Disclosure of potential conflicts of interest is found at the end of this article.

This article has been cited by other articles:

				P. Secchiero, E. Melloni, F. Corallini, A. P. Beltrami, F. Alviano, D. Milani, F. D'Aurizio, M. G. di Iasio, D. Cesselli, G. P. Bagnara, et al. Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Promotes Migration of Human Bone Marrow Multipotent Stromal Cells Stem Cells, November 1, 2008; 26(11): 2955 - 2963. [Abstract] [Full Text] [PDF]

				X. Sang, M. S. Curran, and A. W. Wood Paracrine Insulin-Like Growth Factor Signaling Influences Primordial Germ Cell Migration: In Vivo Evidence from the Zebrafish Model Endocrinology, October 1, 2008; 149(10): 5035 - 5042. [Abstract] [Full Text] [PDF]

				L. da Silva Meirelles, A. I. Caplan, and N. B. Nardi In Search of the In Vivo Identity of Mesenchymal Stem Cells Stem Cells, September 1, 2008; 26(9): 2287 - 2299. [Abstract] [Full Text] [PDF]

				M. R. Loebinger, E. K. Sage, and S. M. Janes Mesenchymal Stem Cells as Vectors for Lung Disease Proceedings of the ATS, August 15, 2008; 5(6): 711 - 716. [Abstract] [Full Text] [PDF]

				A. M. Sonabend, I. V. Ulasov, M. A. Tyler, A. A. Rivera, J. M. Mathis, and M. S. Lesniak Mesenchymal Stem Cells Effectively Deliver an Oncolytic Adenovirus to Intracranial Glioma Stem Cells, March 1, 2008; 26(3): 831 - 841. [Abstract] [Full Text] [PDF]

				S. L. Tomchuck, K. J. Zwezdaryk, S. B. Coffelt, R. S. Waterman, E. S. Danka, and A. B. Scandurro Toll-Like Receptors on Human Mesenchymal Stem Cells Drive Their Migration and Immunomodulating Responses Stem Cells, January 1, 2008; 26(1): 99 - 107. [Abstract] [Full Text] [PDF]

				A. H. Klopp, E. L. Spaeth, J. L. Dembinski, W. A. Woodward, A. Munshi, R. E. Meyn, J. D. Cox, M. Andreeff, and F. C. Marini Tumor Irradiation Increases the Recruitment of Circulating Mesenchymal Stem Cells into the Tumor Microenvironment Cancer Res., December 15, 2007; 67(24): 11687 - 11695. [Abstract] [Full Text] [PDF]

				G. Chamberlain, J. Fox, B. Ashton, and J. Middleton Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing Stem Cells, November 1, 2007; 25(11): 2739 - 2749. [Abstract] [Full Text] [PDF]