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Cardiomyogenic differentiation of human menstrual blood-derived mesenchymal cells (MMCs) was observed in vitro. Immunocytochemistry revealed a clear striation pattern of cardiac troponin-I (red) in about 30% of GFP-positive (green) MMCs. The GFP and troponin-I staining were observed alternately in a striated manner, suggesting that troponin-I is expressed in the GFP-positive cells. See Hida et al. beginning on page 1695.